Two formulations of Guduchi : Preparation and Organoleptic tests

Dr.Vaidehi Patankar, Dr. Madhuri Pawar, Dr Swati Gadgil

Guduchi is one of the “Medhya Rasayan” drug stated in Charak Samhita with its specific form of administration i.e. “Swaras” [Fresh juice].

Many previous research works are there with different forms of Guduchi such as Guduchi Choorna , Kwath or ethanolic and water extractives in which Guduchi Kanda (stem) is used.

Here we have used Fresh Guduchi Kanda (Stem), Patra (Leaves) and Moola(Roots)  

Identification and Authentication of Raw Drug

Whole plant of Guduchi (Tinospora Cordifolia) was used as a Raw Drug.

It was handpicked sample and identified from botany expert from IRSHA, Pune.

Authentication with Macroscopic and Microscopic characters of raw drug was done in IDRAL Laboratory, Pune,

Pharmaceutics of Guduchi Swaras

In present study Guduchi Swaras was prepared as per the standard protocol stated in Sharangdhar Samhita Madhyam Khanda 1^st Chapter verse no.2.

आहतात् तत्क्षणाकृष्टात् द्रव्यात् क्षुण्णात् समुद्धरेत् I

वस्त्र निष्पिडितो यः सः रसः स्वरसः उच्यते II

1. Manufacturing of Guduchi Swaras

2. Analysis of Guduchi Swaras

Method:

Identified and Authenticated Guduchi Whole plant was handpicked from authentic source.

Guduchi Stem having bitter taste was selected for study.Heart shaped green leaf and fresh roots were collected from same plant source.

Preparation of Guduchi swaras:

Standard Operating Procedures were carried out throughout the process.

Ingredients of Gududhci Swaras :’

Sr.No .	Material Used	Part Used	Quantity
1.	Guduchi (Tinospora cordifolia)	Whole plant (Total)	5000gms
1.1	Guduchi stem	Kanda	3500 gms
1.2	Guduchi leaves	Patra	1000 gms
1.3	Guduchi roots	Moola	500 gms
2.	Water	-	1200mL

Instruments:

Mortar-Pestle, Wet Grinder, Measuring cylinder, Iron Vessel, Spoon, Muslin Cloth, Gas Stove

Procedure:

·         Cleaning: Whole plant of Guduchi were separated as Stem, Roots and Leaves and weighed individually. An Individual collected part of plants were washed under running tap water for 3 times.

·         Grinding: The washed stem, small roots and leaves were cut into small pieces and pounded over stony mortar-Pestle. The pounded mixture was put into wet grinder with 25% of water to have moderate rough paste.

·         Filtering:

Obtained rough paste was taken into a clean muslin cloth and tied tightly and it was squeezed to have Guduchi Swaras. It was then measured with measuring cylinder.

·         In process and final testing was done as per the ayurvedic parameters further Guduchi Swaras was analyzed with API parameters.

      Preparation of Guduchi Kalpa:

For better palatability and to increase shelf life of Guduchi swaras “Kalpa” was formulated. It was prepared as per the standard protocol stated for avaleha in  Sharangdhar Samhita Madhyam Khanda 8th chapter verse No.1 as “ Kalpa is to be consider as an advance form of “Avaleha Form”

क्वाथादिनां पुनः पाकाद्घनत्वं सा रसक्रिया I

सोsवलेहश्च लेहः स्यात् तस्य मात्रा पलोन्मिता II 

Table for Quantity of Raw drug 

Sr.No	Raw Drug used	Quantity	Time required to manufacture
1.	Guduchi swaras	350mL	45 Min
2.	Sita (Sugar)	700 gms

ORGNAOLEPTIC CHARACTERISTICS OF GUDUCHI SWARAS

ORGANOLEPTIC TEST
Sr.No.	Parameters	Observations
1.	Shabda	-
2.	Sparsha	Picchila
3.	Roopa	Dark Green colour
4.	Rasa	Tikta
5.	Gandha	Ugra Gandhi

ORGNAOLEPTIC CHARACTERISTICS OF GUDUCHI KALPA

ORGANOLEPTIC TEST
Sr.No.	Parameters	Observations
1.	Shabda	Not applicable
2.	Sparsha	Khar-Sushka
3.	Roopa	Cream  colour
4.	Rasa	Madhur-Tikta
5.	Gandha	Sugandhi

Interpretation:

Handpicked sample of Fresh Guduchi [Tinospora cordifolia] identified and authenticated

Two dosage forms of Guduchi prepared as per standard ayurvedic protocols

Standard operating procedures for Guduchi Swaras and Kalpa are developed.

Organoleptic characteristics of both formulations are estimated